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Purpose We investigated removal of environmental allergenic material derived from Alternaria and Cladosporium using sodium hypochlorite in vivo and in vitro. Methods Freeze dried allergen extract from Alternaria alternata and Cladosporium herbarum was treated with hypochlorite concentrations of88, 38, 16, 3, 0. Remaining native allergenic material was quantified using enzyme immunoassay and remaining viable fungal material was evaluated. Results The results of treating Alternaria or Cladosporium extract with sodium hypochlorite are immediate and obvious.

Concentrations greater than mM remove color and concentrations between and 38 mM partially uncolored the extract. Immunoassay for total antigenic and allergenic material remaining after treatment with sodium hypochlorite including 2 concentrations recommended for killing fungus confirmed a general destruction of antigenic and allergenic material at concentrations of 38 mM or greater. Conclusions This work confirms the ability of solutions of sodium hypochlorite to denature fungal allergenic material from common outdoor and indoor fungi A. Destruction of recognized antigenic and allergenic epitopes occurs at hypochlorite concentrations commonly used for household cleaning.

Cladosporium conidia have been shown to be important aeroallergens in many regions throughout the world, but annual spore concentrations vary considerably between years. Understanding these annual fluctuations may be of value in the clinical management of allergies. This study investigates the number of days in summer when spore concentration exceeds the allergenic threshold in relation to regional temperature and precipitation at two sites in England and Wales over 27 years. Results indicate that number of days in summer when the Cladosporium spores are above the allergenic concentration is positively correlated with regional temperature and negatively correlated with precipitation for both sites over the study period.

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No matter what your taste in pleasure, take your pick of women and men who want to do it with you! The only question is - what kinky fantasies are you ready to live out right now? But remember, because our community is exclusive, we periodically close sign ups to the public. BDSM is almost entirely based around rules, safety and a sense of community. We have rules in place for a reason. So, what are the rules? Everything that goes below this rule is obsolete if communication is poor. No one should launch into a scene without talking it through with the other players first.

Communication is what makes BDSM scenes work so well. You lay out rules and limits beforehand. You reveal medical issues or concerns that might affect play.

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Even light BDSM play has the potential to be incredibly dangerous, both physically and mentally. So before eumsfong, parties should judge what safety measures should be put in place based on the scenario or particular role play being carried out. Things like quick release mechanisms, medical shears, working phone with signal, first aid kits, blankets, sweets or sugary drinks, keys and spares to padlocks and handcuffs, even panic buttons if playing in commercial dungeons. And again, this comes back to thorough communication beforehand.

Any act carried out has to be sane, somehow even though it might not appear that way to others! BDSM players seek reactions, emotions — the more you go into it, the more you want. It can be like a drug. Always stop and think before you try something new:

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